Services Provided and Model Protocol

Patient Derived Xenograft Services offers the following PDTX services:

Engraftment, expansion, characterization and archiving Patient-derived tumor xenografts.

Following receipt of the tumor tissue we will immediately process the tissue for engraftment, cryopreservation and analysis.

Archive—store frozen tissue, FFPE, DNA, RNA.

Implantation and engraftment—typically 2-4 months, possibly up to 6 months.

Analysis—Specific methods of analysis will be discussed with the client prior to performing the analysis.

PDX tumor bearing mice available:

Specific Cancer Models available:

Murine models are described in our science of Patient-Derived Xenografting page here. Some commonly-used models for PDX experiments include:

Clients choose a tumor tissue of interest, an animal (typically immunocompromised murine) model, and work with our experienced scientists to select the number and size of experimental groups. Once in-house, the animals are acclimatized to the vivarium and sorted according to body mass. The selected tissue is thawed and grafted into either the flank area or orthotopically into the area selected by the client. Tumor appearance and other clinical signs are monitored daily, and once tumors appear, the animals are sorted based on tumor volume.

Experimental dosing of desired compounds is initiated either when the mean tumor size reaches a defined volume (known as a staged study) or immediately after the xenograft is inserted (in an unstaged study). The animals are dosed according to the schedule determined by the client, and via client-selected administration route. Administration route options include:

Other administration options available include the frequency of doses, and the duration of the dose administration. Patient Derived Xenograft services also offers a standard positive control option that utilizes cyclophosphamide, at a dosage of 50 mg/kg given by intramuscular injection to the control group daily for the selected study duration.

Experimental studies at Patient Derived Xenograft Services include daily observation of animals by experienced personnel, and typically tumor volume and body weight measurements performed every two days. Additional services available include tumor preservation and other histology, as well as terminal collection of blood sera.